Le malamalama o le phosphoric acid peptide

E faia se sao taua i le faagasologa o le olaga, phosphorylating Tyrr, Ser, Thr, o loʻo i luga o peptides.O le amino acid phosphorylated e masani ona faʻaaogaina i le gaosiga o le phosphoric acid peptide, o loʻo faʻaaogaina nei i le phenyl phosphorylated amino acids.HBTU / phosphorylated amino acids e masani ona faʻaaogaina e faʻafesoʻotaʻi ai le phosphorylated amino acids HOBt / DIEA metotia, ae o le phosphoric acid peptide o loʻo filifilia lenei auala faaletonu, aemaise lava i le gaosia o le peptide peptide umi poʻo le amino acid phosphate, faʻafesoʻotaʻi le lelei e maualalo, maualalo. mama o le oloa mulimuli.Mo lenei phosphopeptide, matou te mafaufau i se auala filifilia post-phosphorylation.O le fa'agasologa o le fa'atupuina o le fa'ate'a'ese'esega lea o vaega fa'apolopolo i le itu o le amino acid e mana'omia ona fa'ailoga pe a mae'a le fa'asologa o le peptide.Mo Tyr, e mafai e Thr ona tali saʻo e faʻaaoga ai amino acids ma filifili itu e le puipuia.-Ser (trt), ave'ese fa'atele ile 1%TFA/DCM si'osi'omaga.

Phosphorylation, faatasi ai ma le lua benzene phosphite phosphoramide ma fa nitrogen ua gaosia ma phosphoramide tetrazolium intermediates galue, fesootai i le hydroxy, ona i lalo o le acid peroxide oxidation phosphoryl fausia, atoatoa tali.

I le taimi nei, o le peptide phosphorylated modification method e lua ituaiga autu:

(1) fa'aofi sa'o ole amino acids phosphorylated fa'amaonia ile fa'asologa o polypeptide;

(2) ina ua maeʻa le resini na gaosia i luga o le peptide sequence, ia Ser, Tyr, poʻo le Thr phosphorylation o le filifili hydroxyl itu.

1) o le a faʻamaonia faʻamaonia phosphorylated amino acids faʻafeiloaʻi saʻo le faasologa peptide:

O lona uiga, o amino acids e manaʻomia le phosphorylated o le a phosphorylated ma puipuia lelei i luma o le faʻaaogaina (Thr,Ser poʻo Tyr), ona faʻaititia ai lea o le phosphorylation i le tulaga filifilia o le polypeptide index e faʻavae i luga ole faagasologa masani ole gaosiga SPPS. .O lenei auala e faigofie ona faagaoioia, ua avea ma auala autu o pisinisi peptide phosphorylated suiga.

2) pe a maeʻa le resini na gaosia i luga o le peptide sequence, i le Ser hydroxy phosphorylation, Tyr, poʻo le Thr itu filifili:

Phosphorylation teuteuga e faʻaaoga ai le phosphorylation o se faʻaupuga e tasi i le auala polypeptide, ona o le faʻalavelave faʻalavelave faʻatupuina e vaega tetele i le teuteuga filifili itu, e faigata mo le phosphorylation o amino acids e faʻaitiitia i le filifili peptide.I le lumanaʻi, o le faʻaofiina o amino acids o le a sili atu ona faigata, aemaise lava i le teuteuga o loʻo i ai le tele o nofoaga o le phosphorylation, o le a matua faigata lava le augatupulaga, o le gaosiga o oloa mulimuli e faigata, faigata ona vavae ese, ma o le fua e matua maualalo lava.

O le mea lea, pe a phosphorylated le tele o nofoaga i le filifili peptide, e mafai e se tasi ona filifili e gaosia le faasologa peptide i luga o le resin ona phosphorylate ai e le vaega filifili hydroxyl vaega o Ser, Tyr, po o Thr: o lona augatupulaga e aofia ai le filifilia o le aveesea o le. vaega puipui filifili filifili e manaʻomia le amanaʻiaina o amino acids pe a uma le peptide synthesis.Mo Tyr, e mafai e Thr ona faʻaaoga saʻo le amino acid filifili filifili e le puipuia i le tali.

Itu faavae puipuiga filifili 1% TFA/DCM siosiomaga mafai quantitatively aveesea.A filifilia lenei metotia, e mafai ona gaosia se phosphoramidite tetrazolium active intermediate e faʻaaoga ai le dibenzyl phosphoramidite ma le tetrazolium, faʻafesoʻotaʻi i se vaega hydroxyl, ona faʻamaʻiina lea e fausia ai se vaega phosphoryl i le peroxyacid environment e faʻamaeʻa ai le tali.