Auala o le aveeseina
I le 1950s ma le 1960s, o le tele o atunuu i le lalolagi, e aofia ai Saina, e masani lava ona aumaia peptides mai totoga o manu.Mo se faʻataʻitaʻiga, o le tui o le thymosin e saunia e ala i le fasiina o se tamai povi faatoa fanau mai, aveese lona thymus, ona faʻaaogaina lea o le biotechnology o le oscillating separation e vavae ese ai peptides mai le tamaʻi povi.O lenei thymosin o loʻo faʻaaogaina lautele e faʻatonutonu ma faʻaleleia le faʻaogaina o le tino i totonu o tagata.
O peptides fa'aanatura fa'aanatura e fa'asalalau lautele.E tele peptides bioactive i manu, laau ma meaola o le gataifale i le natura, o loʻo taʻalo ai le tele o galuega faʻaletino ma tausia gaioiga masani o le olaga.O nei peptides fa'ale-natura e aofia ai metabolites lona lua o meaola e pei o vailaʻau faʻamaʻi ma hormones, faʻapea foʻi ma bioactive peptides o loʻo i ai i totonu o masini eseese.
I le taimi nei, e tele peptides bioactive ua vavae ese mai tagata, manu, laau, microbial ma meaola o le gataifale.Ae ui i lea, o peptides bioactive e masani lava ona maua i ni aofaiga maualalo i meaola, ma o auala o loʻo i ai nei mo le tuʻufaʻatasia ma le faʻamamaina o peptides olaola mai meaola faanatura e le atoatoa, ma le tau maualuga ma le maualalo o le bioactivity.
O auala masani e faʻaaogaina mo le faʻaaogaina o le peptide ma le vavaeeseina e aofia ai le salting out, ultrafiltration, gel filtration, isoelectric point precipitation, ion exchange chromatography, affinity chromatography, adsorption chromatography, gel electrophoresis, ma isi.
Metotia fa'avae acid
Acid ma alkali hydrolysis e masani ona faʻaaogaina i faʻalapotopotoga faʻataʻitaʻi, ae e seasea faʻaaogaina i le gaosiga o galuega.I le faagasologa o le alkaline hydrolysis o polotini, o le tele o amino acids e pei o le serine ma le threonine e faʻaumatia, e tupu ai le racemization, ma le tele o meaʻai e leiloa.O le mea lea, o lenei metotia e seasea faʻaaogaina i le gaosiga.Acid hydrolysis o polotini e le mafua ai le racemization o amino acids, hydrolysis e vave ma atoatoa le tali.Ae ui i lea, o ona tulaga le lelei o tekinolosi lavelave, faigata le pulea ma le ogaoga o le faaleagaina o le siosiomaga.O le tufatufaina atu o le mamafa o peptide e le tutusa ma le mautu, ma o latou galuega faʻaletino e faigata ona fuafua.
Enzymatic hydrolysis
Ole tele ole bioactive peptides o lo'o maua ile filifili uumi ole polotini ile tulaga le toaga.Pe a faʻasusuina e se protease faʻapitoa, o latou peptide malosi e faʻamalolo mai le faʻasologa o amino o le porotini.Ole su'esu'eina ole Enzymatic ole bioactive peptides mai manu, la'au ma meaola o le gataifale ose su'esu'ega taula'i ile sefulu tausaga talu ai.
Enzymatic hydrolysis o bioactive peptides o le filifilia lea o protease talafeagai, faʻaaogaina polotini e fai ma substrates ma hydrolyzing proteins e maua ai le tele o peptides bioactive ma galuega faʻaletino eseese.I le faagasologa o le gaosiga, vevela, tau PH, enzyme concentration, substrate concentration ma isi mea taua e vavalalata vavalalata ma le aafiaga hydrolysis enzymatic o peptides laiti, ma o le ki o le filifiliga o le enzyme.Ona o le eseese o enzymes e faʻaaogaina mo le enzymatic hydrolysis, o le filifilia ma le faʻatulagaina o enzymes, ma punaoa eseese o porotini, o peptides e maua mai e matua ese lava i le tele, tufatufaina o le mamafa o le mole, ma le tuufaatasiga o le amino acid.E masani ona filifilia e se tasi ia mea e puipuia ai manu, e pei o le pepsin ma le trypsin, ma le protease o laau, e pei o le bromelain ma le papain.Faatasi ai ma le atinaʻeina o saienisi ma tekonolosi ma le faʻaauauina o le faʻafouina o le biological enzyme technology, o le a sili atu ma sili atu enzymes o le a maua ma faʻaaogaina.Enzymatic hydrolysis ua faʻaaogaina lautele i le sauniuniga o peptides bioactive ona o lona tekinolosi matua ma le maualalo o tupe teufaafaigaluega.
Taimi meli: Me-30-2023