Suiga o le methylation

Methylation-modified peptides, e taʻua foi o methylation-recognized peptides, o polotini pe a maeʻa faʻaliliuga teuteu (PTMs) ma faia se matafaioi faʻatulafonoina i le toetoe o gaioiga uma o le olaga i sela.O polotini e fa'apipi'iina e le methyltransferase e fa'afeiloa'i vaega o le hydroxyl i toega amino acid fa'apitoa mo le fusifusia fa'atasi.O le Methylation o se fa'agasologa o suiga e mafai ona fa'aaogaina e demethylases.

O suʻesuʻega ua faʻaalia ai o le methylated / demethylated amino acids masani e masani ona lysine (Lys) ma arginine (Arg).O suʻesuʻega ua maua ai o le histone lysine methylation o loʻo i ai le tele o galuega faʻapitoa e pei o le tausiga o le cell cell ma le vaevaega, X chromosome inactivation, faʻasologa o tusitusiga ma tali faʻaleagaina DNA.", e masani ona aʻafia ai le chromatin condensation ma taofiofia le faʻaaliga o gene."O le Histone arginine methylation e taua tele i le faʻatonutonuina o le faʻasologa o gene ma e mafai ona aʻafia ai faiga faʻaletino eseese i sela, e aofia ai le toe faʻaleleia o le DNA, faʻaliliuga faʻailoga, atinaʻeina o sela, ma le carcinogenesis.O le mea lea, o le Guopeptide Biology ua faʻapitoa ona atiaʻe le tekonolosi o methyl decorative peptides, lea e faʻaleleia e saienitisi ina ua maeʻa le faʻaliliuga o le protein (PTMS) e fesoasoani i le suʻesuʻega.

Suiga Methylation (Me1, Me2, Me3)

Tulaga maualuga Fmoc-Lys(Me,Boc)-OH, Fmoc-Lys(Me2)-OH, Fmoc-Lys(Me3)-OH.HCL, Fmoc-Arg(Me,Pbf)-OH, Fmoc-Arg(Me) 2-OH.HCl(asymmetrical), F na faʻaaogaina moc-Arg (me) 2-OH.HCl (symmetrical) ma isi mea mata na gaosia e le FMOC solid-phase synthesis process e maua ai Lys ma Arg methylated peptides, ma oloa. na faamamaina e le HPLC.Ole tele ole spectra talafeagai, HPLC chromatograms ma COA o loʻo tuʻuina atu mo le oloa maeʻa.